GW: Gateway Summary
FLJ32057AAAF
Peptide Sequence (833 aa)
MTWGALFRHEILSRDPLLLAIIPKYLRASMTNLVKMGFPSKTDSPSCEYSRFDFDSDEDF NAFFNSSRAQQGEVMRLACRLDPKTSFQMAGEWLKYQLSTFLDAGSVNSCSAVGTGEGSL CSVFSPSFVQWEAMTLFLESVITQMFRTLNREEIPVNDGIELLQMVLNFDTKDPLILSCV LTNVSALFPFVTYRPEFLPQVLSKLFSSVTFETVEESKAPRTRAVRNVRRHACSSIIKMC RDYPQLVLPNFDMLYNHVKQLLSNELLLTQMEKCALMEALVLISNQFKNYERQKVFLEEL MAPVASIWLSQDMHRVLSDVDAFIAYVGTDQKSCDPGLEDPCGLNRARMSFCVYSILGVV KRTCWPTDLEEAKAGGFVVGYTSSGNPIFRNPCTEQILKLLDNLLALIRTRNTLYAPEML AKMAEPFTKALDMLDAEKSAILGLPQPLLELNDSPVFKTVLERMQRFFSTLYENCFHILG KAGPSMQQDFYTVEDLATQLLSSAFVNLNNIPDYRLRPMLRVFVKPLVLFCPPEHYEALV SPILGPLFTYLHMRLSQKWQVINQRSLLCGEDEAADENPESQEMLEEQLVRMLTREVMDL ITVCCVSKKGADHSSAPPADGDDEEMMATEVTPSAMAELTDLGKCLMKHEDVCTALLITA FNSLAWKDTLSCQRTTSQLCWPLLKQVLSGTLLADAVTWLFTSVLKGLQMHGQHDGCMAS LVHLAFQIYEALRPRYLEIRAVMEQIPEIQKDSLDQFDCKLLNPSLQKVADKRRKDQFKR LIAGCIGKPLGEQFRKEVHIKNLPSLFKKTKPMLETEVLDNDGGGLATIFEPY
Nucleotide Sequence (2,499 bp)
ATGACTTGGGGAGCCCTCTTCAGGCATGAAATCCTGTCCCGTGATCCTTTGCTATTAGCA ATAATACCAAAATATCTTCGTGCTTCCATGACTAACTTGGTCAAGATGGGCTTTCCTTCT AAAACAGACAGCCCTAGCTGTGAATATTCTCGGTTTGATTTTGATAGCGATGAGGACTTC AATGCTTTCTTCAACTCCTCCCGAGCACAACAAGGAGAGGTGATGAGGTTGGCATGTCGT TTGGATCCCAAAACTAGCTTCCAGATGGCTGGGGAGTGGCTAAAGTATCAACTATCAACT TTTCTTGATGCTGGTTCTGTGAATTCTTGTTCTGCAGTTGGAACTGGAGAAGGAAGCCTC TGTTCCGTCTTCTCACCTTCATTCGTGCAGTGGGAAGCCATGACTCTTTTTTTGGAAAGT GTTATCACCCAGATGTTTCGAACACTAAATAGAGAAGAAATTCCTGTTAATGATGGAATA GAGCTATTGCAGATGGTTCTGAACTTTGATACCAAGGATCCCCTCATCCTGTCCTGCGTC CTTACTAATGTCTCTGCACTCTTTCCATTTGTCACCTACAGACCAGAGTTCCTGCCCCAG GTCCTCTCTAAGCTATTTTCATCTGTCACTTTTGAAACTGTTGAAGAAAGTAAGGCCCCC AGAACCCGGGCAGTGAGGAATGTGAGGAGGCATGCTTGTTCCTCCATCATCAAGATGTGT CGTGACTACCCCCAGCTTGTGCTGCCCAATTTTGACATGCTTTATAACCATGTGAAGCAA CTCCTCTCCAATGAGCTACTCCTGACACAAATGGAGAAGTGTGCCCTCATGGAAGCCCTG GTTCTCATTAGCAACCAATTTAAGAACTACGAGCGTCAGAAGGTGTTCCTAGAGGAGCTG ATGGCACCAGTGGCCAGCATCTGGCTTTCTCAAGACATGCACAGAGTGCTGTCAGATGTT GATGCTTTCATTGCGTATGTGGGTACAGATCAGAAGAGCTGTGACCCAGGCCTGGAGGAT CCGTGTGGCTTAAACCGTGCACGAATGAGCTTTTGTGTATACAGCATTCTGGGTGTGGTG AAACGAACTTGCTGGCCCACTGACCTAGAAGAGGCCAAAGCTGGGGGATTTGTGGTGGGT TATACATCCAGTGGAAATCCAATCTTCCGTAACCCCTGCACAGAGCAGATTCTGAAACTT CTTGACAATTTGCTTGCGCTTATAAGAACCCGCAATACATTATATGCACCAGAAATGCTA GCCAAAATGGCAGAGCCTTTCACCAAGGCTCTGGATATGCTTGACGCGGAAAAATCTGCT ATATTAGGATTACCTCAACCTCTCTTGGAACTCAATGACTCTCCTGTCTTCAAAACCGTC TTGGAAAGAATGCAGCGTTTCTTCTCTACCCTCTATGAAAACTGTTTTCATATCCTAGGG AAGGCAGGCCCTTCCATGCAGCAAGACTTCTATACTGTGGAGGACCTTGCTACCCAGCTT CTCAGCTCAGCCTTTGTCAACTTGAACAATATTCCTGACTACCGACTCAGACCCATGCTT CGTGTCTTTGTAAAGCCTCTGGTGCTCTTCTGTCCCCCAGAGCACTATGAAGCCCTGGTA TCCCCCATCCTCGGACCTCTTTTCACCTACCTCCATATGAGGCTTTCTCAGAAATGGCAA GTTATCAACCAAAGGAGCCTGCTGTGTGGAGAAGATGAGGCTGCAGATGAAAACCCAGAG TCTCAAGAGATGCTGGAGGAGCAACTGGTGAGGATGTTAACCCGAGAAGTCATGGACCTA ATCACGGTTTGCTGTGTTTCAAAGAAGGGTGCTGACCACAGTAGTGCTCCCCCAGCAGAT GGAGACGATGAAGAAATGATGGCCACAGAGGTCACCCCCTCAGCTATGGCAGAGCTTACA GACCTGGGCAAATGTCTGATGAAGCATGAGGATGTTTGTACAGCGCTATTAATTACAGCC TTCAATTCCCTGGCCTGGAAAGATACTCTGTCCTGCCAGAGGACAACCTCACAGCTCTGC TGGCCTCTCCTCAAACAAGTGCTGTCAGGGACACTGCTCGCAGATGCAGTTACGTGGCTT TTCACCAGTGTGCTGAAAGGCTTACAGATGCACGGGCAGCACGACGGGTGCATGGCTTCC CTGGTCCATCTGGCCTTCCAGATATATGAGGCACTGCGCCCCAGGTACCTGGAGATAAGA GCTGTAATGGAGCAAATCCCTGAAATACAGAAGGACTCACTGGACCAGTTTGACTGCAAG CTTTTAAACCCCTCCCTGCAGAAAGTGGCTGACAAGCGCCGAAAGGACCAATTCAAACGC CTCATTGCTGGTTGCATTGGGAAACCCTTGGGAGAGCAGTTCCGAAAAGAAGTTCACATT AAGAATCTTCCCTCACTTTTCAAAAAAACAAAGCCAATGCTGGAGACGGAGGTGCTGGAC AATGATGGGGGTGGCCTGGCCACCATCTTTGAACCCTAT
Sequence Difference from Target Sequence
>AK056619.1 (653..3151)
A1232G:CAC(His)CGC(Arg)
PE: Protein Expression
| Data Type | Expression System | Destination Vector | Additional Tag | Result |
|---|---|---|---|---|
| Experiment | Wheat germ cell-free system | pEW-3H | C-terminal 6xHis | T:0,S:0 |
SL: Subcellular Localization
| Data Type | Transfected Cell | Destination Vector | Additional Tag | Result |
|---|---|---|---|---|
| Experiment | HeLa cell | pDEST-MCEYFP | C-terminal EYFP | Under evaluation |
